Pulsed EPR spectroscopy distance measurements of DNA internally labelled with Gd(3+)-DOTA.
نویسندگان
چکیده
Gd(3+) is increasingly used in EPR spectroscopy due to its increased intracellular stability and signal-to-noise ratios. Here we present the incorporation of Gd(3+)-DOTA into internal positions in DNA. Distance measurements via pulsed Electron Paramagnetic Resonance (EPR) spectroscopy in vitro and in cellula proved enhanced stability and efficiency compared to nitroxide labels.
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عنوان ژورنال:
- Chemical communications
دوره 51 72 شماره
صفحات -
تاریخ انتشار 2015